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Difference between revisions of "Liposome Extruders"
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The multiple liposome extruders system is able to process the volume from 1L to 200L. It features both the temperature and pressure sensors in the product line, and a control panel to control the production for liposomes. | The multiple liposome extruders system is able to process the volume from 1L to 200L. It features both the temperature and pressure sensors in the product line, and a control panel to control the production for liposomes. | ||
=Track-Etched Polycarbonate Extrusion Membranes= | |||
[[File:Microscopic_Representation_for_Track-Etched_Polycarbonate_Membranes.png|thumb|250px|right|Microscopic Representation for Track-Etched Polycarbonate Membranes]] | |||
The operations of liposome extruders have high requirements for the extrusion membranes. The nucleus track-etched polycarbonate membranes should have uniform distributions of filter pores. In an ideal membrane, all nano pores should be of the same size. Furthermore, the extrusion membrane with vertical pore distribution is more conducive to the preparation of liposome extrusion. | |||
In general, selecting right size of the polycarbonate membranes will ensure successful extrusion. It is strongly recommended to know the initial particle size of the processing sample before the extrusion. Another factor to consider for selecting the membrane is the desired particle size for your end product. | |||
For example, if the initial particle size of the sample is 1μm, and the target is to achieve the particle size below 100 nm, here are several options available: | |||
==Extrusion Multiple Times via the Target Pore Size Membranes== | |||
For example, the user can extrude the sample through a 100 nm polycarbonate membrane using a liposome extruder several times. | |||
But the problems that may arise are: | |||
* The difficulties in extrusion process; | |||
* The large vesicles may block the surface of the polycarbonate membrane, and request a replacement of a new membrane, etc. | |||
==Extrusion via the Different Pore Size Membranes== | |||
This procedure is to use different pore size membranes to extrude step by step. For example, the sample is extruded through a 400nm polycarbonate membrane first, and observe the extrusion process. If it is hard to extrude the sample through the 400nm membranes, then replace a new membrane with a larger pore size, such as 800nm for extrusion 3~5 times. It can be optimized during the process. After the first-round extrusion, select a smaller pore size polycarbonate membrane, such as 100 nm or 80 nm to extrude again. 200 nm polycarbonate membranes can be used as a transition if the extrusion is difficult to go through the 100nm membranes. | |||
==High Pressure Homogenization-Extrusion Method== | |||
[[File:High_Pressure_Homogenizer_combines_with_online_liposome_extruder.png|thumb|250px|right|High Pressure Homogenizer combines with online liposome extruder]] | |||
The sample can be high pressure homogenized first, and extruded through a 100nm or 80nm polycarbonate membrane after homogenization. | |||
=Videos= | =Videos= | ||